Effect of the bacterial growth rate on replication control of plasmid pBR322 in Escherichia coli
β Scribed by Lin-Chao, S. ;Bremer, H.
- Publisher
- Springer
- Year
- 1986
- Tongue
- English
- Weight
- 676 KB
- Volume
- 203
- Category
- Article
- ISSN
- 0026-8925
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The in vivo role of the Escherichia coli protein DnaA in the replication of plasmid pBR322 was investigated, using a plasmid derivative carrying an inducible dnaA+ gene. In LB medium without inducer, the replication of this plasmid, like that of pBR322, was inhibited by heat inactivation of chromoso
The replication pattern of the plasmid pBR322 was examined in the dnaA mutants of Escherichia coli. The rate of pBR322 DNA synthesis is markedly decreased after dnaA cells are shifted to the restrictive temperature of 42 degrees C. However, addition of rifampicin (RIF) to cultures of dnaA strains in