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Effect of the bacterial growth rate on replication control of plasmid pBR322 in Escherichia coli

✍ Scribed by Lin-Chao, S. ;Bremer, H.


Publisher
Springer
Year
1986
Tongue
English
Weight
676 KB
Volume
203
Category
Article
ISSN
0026-8925

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The replication pattern of the plasmid pBR322 was examined in the dnaA mutants of Escherichia coli. The rate of pBR322 DNA synthesis is markedly decreased after dnaA cells are shifted to the restrictive temperature of 42 degrees C. However, addition of rifampicin (RIF) to cultures of dnaA strains in