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Amplification of different ColEl plasmids in an Escherichia coli relA strain

✍ Scribed by Dr. A. Schroeter; S. Riethdorf; M. Hecker

Publisher: John Wiley and Sons
Year: 1988
Tongue: English
Weight: 233 KB
Volume: 28
Category: Article
ISSN: 0233-111X
DOI: 10.1002/jobm.3620280818

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✦ Synopsis

Amino acid starved cells of an E. coli relA strain accumulate a large amount of pBR322 plasmid DNA. In this study ColEl related plasmids of different copy number and size including a high copy number plasmid mutant of pBR322 were amplified in a relA strain of E. coli K-12 under amino acid limitation in order to determine the upper plasmid level in amino acid starved cells. In all cases we measured a 4 to 6 fold increase of the plasmid copy number in comparison to log-phase cells independent of the size, the number of origins per plasmid molecule or the copy number in log-phase cells. The plasmid copy number in amino acid starved cells varies from about 200 (pBR322-dimer) to about 2000 (high copy number plasmid pERIII-BPL4, see BOROS et al. 1986). Rop' and rop-plasmids show the same amplification rate under the used conditions.

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