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Peptide sequencing through N-terminal phosphonylation and electrospray ionization mass spectrometry

✍ Scribed by Jiangyin Bao; Huiwang Ai; Hua Fu; Yuyang Jiang; Yufen Zhao; Cheng Huang

Publisher
John Wiley and Sons
Year
2005
Tongue
English
Weight
125 KB
Volume
40
Category
Article
ISSN
1076-5174

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✦ Synopsis

Abstract

Peptides were phosphonylated at their N‐termini by reacting with ethoxyphenylphosphinate in the presence of triethylamine and tetrachloromethane under mild conditions. The phosphonylated peptides were analyzed by tandem electrospray ionization mass spectrometry. N‐Terminal phosphonylation selectively increased the intensities of b~n~‐type ions relative to other ion types. The resulting simplified mass spectra clearly show the sequential loss of amino acid residues from the C‐termini of peptides, providing a convenient and rapid method for peptide sequencing. Copyright Β© 2005 John Wiley & Sons, Ltd.

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