Crude mixtures of difficult tetrapeptide cyclization reactions were analyzed by electrospray ionization mass spectrometry to optimize the production of cyclomonomer versus cyclodimer. The cationization phenomenon enabled the differentiation between monoprotonated and multiply charged ions of the sam
Antisense peptide interactions studied by electrospray ionization mass spectrometry
β Scribed by Madhusudanan, K. P.; Katti, S. B.; Haq, W.; Misra, P. K.
- Publisher
- John Wiley and Sons
- Year
- 2000
- Tongue
- English
- Weight
- 85 KB
- Volume
- 35
- Category
- Article
- ISSN
- 1076-5174
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β¦ Synopsis
Non-covalent interactions between met-and leu-enkephalins and their antisense peptides were studied by electrospray ionization mass spectrometry. Mixtures of sense and antisense peptides gave both the corresponding homodimers and heterodimers. The relative abundance ratios of the heterodimer to that of the homodimer of the sense peptide and the relative stability constants of the heterodimers were compared with the corresponding values from mixtures of the sense peptides and a control peptide. The results show that there is a preferential interaction between the sense and antisense peptides compared with that between the sense and control peptides.
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