Partial purification and properties of pectin lyase fromPenicillium expansum

✍ Scribed by D. O. Silva; M. M. Attwood; D. W. Tempest

• Book ID: 104749960
• Publisher: Springer
• Year: 1993
• Tongue: English
• Weight: 511 KB
• Volume: 9
• Category: Article
• ISSN: 1573-0972
• DOI: 10.1007/bf00386297

✦ Synopsis

A pectin lyase, poly(methoxygalacturonide) lyase, EC 4.2.2.10, from a culture filtrate of Penicillium expansum was partially purified 33-fold with 7.3% yield. The enzyme was monomeric with a molecular mass of 36.5 kDa. The enzyme did not contain pectate lyase activity and degraded citrus and apple pectin best at pH 7.0 and 40 to 45°C. The K m for citrus pectin was 9 mg ml(-1).