Purification and characterization of pectin lyase secreted byPenicillium citrinum
β Scribed by S. Yadav; P. K. Yadav; D. Yadav; K. D. S. Yadav
- Book ID
- 110155204
- Publisher
- Springer
- Year
- 2009
- Tongue
- English
- Weight
- 460 KB
- Volume
- 74
- Category
- Article
- ISSN
- 0006-2979
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Mycelial growth and production of extracellular pectin lyase by Penicillium griseoroseum at different concentrations of inducers were investigated. The fungus was cultured in mineral medium using sucrose as a carbon source and caffeine, yeast extract, tea extract or pectin as inducers. Caffeine, yea
A pectin lyase, poly(methoxygalacturonide) lyase, EC 4.2.2.10, from a culture filtrate of Penicillium expansum was partially purified 33-fold with 7.3% yield. The enzyme was monomeric with a molecular mass of 36.5 kDa. The enzyme did not contain pectate lyase activity and degraded citrus and apple p
Optimum activity of an extracellular pectin lyase produced by Penicillium griseoroseum in submerged culture was after 120 h using 0.1% (w/v) citrus pectin as substrate. Sucrose at 0.1% (w/v) stimulated enzyme production and citrus pectin gave the highest activity of enzyme per unit growth.