๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

Partial Purification and Properties of a Bacterial Isoamylase

โœ Scribed by Richards M. Evans; David J. Manners; J.Roger Stark

Book ID
102990971
Publisher
Elsevier Science
Year
1979
Tongue
English
Weight
697 KB
Volume
76
Category
Article
ISSN
0008-6215

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โœฆ Synopsis

Isoamylase has been prepared by affinity chromatography of a commercial enzyme-preparation from a strain of Cytophaga (also known as a Flavobacterium or Polyangium). The enzyme was not very stable, but the stability could be improved by calcium ions. The enzyme had a very low but significant activity on pullulan and on alpha-dextrins having maltosyl side-chains. This observation, which is contrary to previous reports, has been related to the specificity of isoamylase and other bacterial debranching-enzymes.

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