✦ LIBER ✦

Partial purification and some properties of lipoxygenase fromSaccharomyces cerevisiae

✍ Scribed by B. Bisakowski; S. Kermasha; C. Schuepp

Publisher: Springer
Year: 1995
Tongue: English
Weight: 236 KB
Volume: 11
Category: Article
ISSN: 1573-0972
DOI: 10.1007/bf00286359

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✦ Synopsis

A partially purified lipoxygenase extract was obtained from the yeast Saccharomyces cerevisiae by precipitation with solid (NH4)SO4 at 20% to 80% saturation. The enzyme had two pH optima, at pH 8.0 and 10.0, with respective apparent K m values of 13 and 9.5 μM. At both pH optima, the lipoxygenase demonstrated highest substrate specificity towards linoleic acid, followed by linolenic acid; although the enzyme had less specificity towards mono-linolein than di-linolein at pH 8.0, the reverse was true at pH 10.0.

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