✦ LIBER ✦

Purification and properties of polyphosphatase fromSaccharomyces cerevisiae cytosol

✍ Scribed by Andreeva, Nadezhda; Kulakovskaya, Tatjana; Sidorov, Igor; Karpov, Alexander; Kulaev, Igor

Publisher: John Wiley and Sons
Year: 1998
Tongue: English
Weight: 144 KB
Volume: 14
Category: Article
ISSN: 0749-503X
DOI: 10.1002/(sici)1097-0061(19980315)14:4<383::aid-yea232>3.0.co;2-s

No coin nor oath required. For personal study only.

✦ Synopsis

A homogenous polyphosphatase preparation was obtained from Saccharomyces cerevisiae cytosol with a 3•8% yield and 3540-fold purification. The enzyme hydrolysed polyphosphate (polyP) with various chain lengths, including polyP 3 , and split P i off the end of the chain. It was inactive with respect to ATP, PP i , and p-nitrophenylphosphate. Its specific activity with polyP 15 was 283 U/mg protein. The polyphosphatase was a monomeric protein with a molecular mass of 40 kDa. This enzyme was inactive without divalent cations and with Cu 2+ and Ca 2+ . The ability of other divalent cations to activate the enzyme decreased in the following order: Co 2+ >Mn 2+ >Mg 2+ >Zn 2+ . A kinetic model of the hydrolysis of polyP 3 and action of Mg 2+ is proposed. 1998 John Wiley & Sons, Ltd.

📜 SIMILAR VOLUMES

Purification of Saccharomcyes cerevisiae

Purification of Saccharomcyes cerevisiae Mitochondria Devoid of Microsomal and Cytosolic Contaminations

✍ Chris Meisinger; Thomas Sommer; Nikolaus Pfanner 📂 Article 📅 2000 🏛 Elsevier Science 🌐 English ⚖ 183 KB

and NeuAc. In Fig. , DPA revealed all monosaccharides analyzed. In this case, mobility and color could be used to distinguish specific sugars. Colors of different monosaccharides shown in Fig. varied from light blue to pink, gray, and purple. As shown in Fig. , color formation of chromatograms spra

Identification and characterization of i

Identification and characterization of individual cyclin-dependent kinase complexes fromSaccharomyces cerevisiae

✍ Shellman, Yiqun G.; Svee, Eric; Sclafani, Robert A.; Langan, Thomas A. 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 415 KB

In S. cerevisiae, regulation of cell cycle progression is known to be carried out by a single cyclin-dependent kinase homologue, Cdc28p, acting at different stages of the cell cycle in association with various cyclins and other regulatory subunits. However, a still unsolved problem is the identifica

Functional analysis of 12 ORFs fromSacch

Functional analysis of 12 ORFs fromSaccharomyces cerevisiae chromosome II

✍ Rodríguez-Navarro, S.; Estruch, F.; Pérez-Ortin, J. E. 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 231 KB 👁 2 views

Twelve different ORFs have been deleted from the right arm of Saccharomyces cerevisiae chromosome II; namely YBR193c, YBR194w, YBR197c, YBR198c, YBR201w, YBR203w, YBR207w, YBR209w, YBR210w, YBR211c, YBR217w and YBR228w. Tetrad analysis of heterozygous deletant strains revealed that YBR193c, YBR198c

Sequence Analysis of 203 Kilobases fromS

Sequence Analysis of 203 Kilobases fromSaccharomyces cerevisiae Chromosome VII

✍ Rieger, Michael; Brückner, Margit; Schäfer, Melanie; Müller-Auer, Silke 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 160 KB 👁 2 views

The nucleotide sequences of five major regions from chromosome VII of Saccharomyces cerevisiae have been determined and analysed. These regions represent 203 kilobases corresponding to approximately one-fifth of the complete yeast chromosome VII. Two fragments originate from the left arm of this chr

Crystallization of the NAD-dependent 5,1

Crystallization of the NAD-dependent 5,10-methylenetetrahydrofolate dehydrogenase fromSaccharomyces cerevisiae

✍ Monzingo, Arthur F.; West, Mary G.; Schelp, Elisabeth; Appling, Dean R.; Robertu 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 English ⚖ 189 KB

## Saccharomyces cerevisiae possesses three isozymes of 5,lO-methylenetetrahydrofolate dehydrogenase (MTD). The NADdependent enzyme is the first monofunctional form found in eukaryotes. Here we report its crystallization in a form suitable for high-resolution structure. The space group is P4,2,2 w

Disruption and basic functional analysis

Disruption and basic functional analysis of six novel ORFs of chromosome XV fromSaccharomyces cerevisiae

✍ Lafuente, Maria J.; Gancedo, Carlos 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 303 KB 👁 2 views

We report the disruption and functional analysis of six open reading frames (ORFs) from chromosome XV, namely YOL155c, YOL154w, YOL119c, YOL118c, YOR301w and YOR306c, in FY1679 and CEN.PK2 backgrounds. We constructed replacement cassettes and cloned each ORF into the pRS416 centromeric plasmid. No o