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Functional analysis of 12 ORFs fromSaccharomyces cerevisiae chromosome II

✍ Scribed by Rodríguez-Navarro, S.; Estruch, F.; Pérez-Ortin, J. E.


Publisher
John Wiley and Sons
Year
1999
Tongue
English
Weight
231 KB
Volume
15
Category
Article
ISSN
0749-503X

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✦ Synopsis


Twelve different ORFs have been deleted from the right arm of Saccharomyces cerevisiae chromosome II; namely YBR193c, YBR194w, YBR197c, YBR198c, YBR201w, YBR203w, YBR207w, YBR209w, YBR210w, YBR211c, YBR217w and YBR228w. Tetrad analysis of heterozygous deletant strains revealed that YBR193c, YBR198c and YBR211c are essential genes for vegetative growth. No effects were detected in any of the haploid deletion mutants for the rest of the ORFs with respect to growth, gross morphology or mating.


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