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Construction and genetic analysis ofS. cerevisiae deletants of six novel ORFs from chromosome II

✍ Scribed by Malagón, Francisco; Aguilera, Andrés

Publisher
John Wiley and Sons
Year
1999
Tongue
English
Weight
223 KB
Volume
15
Category
Article
ISSN
0749-503X

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✦ Synopsis

We have constructed S. cerevisiae strains carrying genomic deletions of six ORFs from the left arm of chromosome II (YBL018c, YBL019w, YBL024w, YBL042c, YBL043w and YBL046w) in both FY1679 and W303 backgrounds. We have found that YBL018c is an essential gene in yeast, whereas the other five genes are non-essential. We have developed plasmids carrying deletion cassettes that can be used to delete any of the six genes in S. cerevisiae by transforming to G418-resistance, as well as centromeric plasmids containing the cognate genes.

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