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Functional Analysis of six novel ORFs on the left arm of Chromosome XII of Saccharomyces cerevisiae reveals three of them responding to S-starvation

✍ Scribed by Nianshu Zhang; Cristina Merlotti; Jian Wu; Thamir Ismail; Abdel-Nasser El-Moghazy; Shakeel Ahmed Khan; Amna Butt; David C. J. Gardner; Paul F. G. Sims; Stephen G. Oliver

Publisher: John Wiley and Sons
Year: 2001
Tongue: English
Weight: 184 KB
Volume: 18
Category: Article
ISSN: 0749-503X
DOI: 10.1002/1097-0061(20010315)18:4<325::aid-yea669>3.0.co;2-k

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Deletion via PCR-mediated gene replacement, together with basic functional and bioinformatic analyses, have been performed on six novel open reading-frames (ORFs) on the left arm of chromosome XII of Saccharomyces cerevisiae (YLL033w, YLL032c, YLL031c, YLL030c, YLL029w and YLL028w). ORF deletion was

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Six novel Open Reading Frames (ORFs) located on the left arm of chromosome XII (YLL044w, YLL042c, YLL040c, YLL038c, YLL035w and YLL034c) have been analysed using short-¯anking homology (SFH) gene replacement. Sporulation and tetrad analysis showed that YLL035w and YLL034c are essential for cell grow