𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Disruption and basic functional analysis of six novel ORFs of chromosome XV fromSaccharomyces cerevisiae

✍ Scribed by Lafuente, Maria J.; Gancedo, Carlos

Publisher
John Wiley and Sons
Year
1999
Tongue
English
Weight
303 KB
Volume
15
Category
Article
ISSN
0749-503X

No coin nor oath required. For personal study only.

✦ Synopsis

We report the disruption and functional analysis of six open reading frames (ORFs) from chromosome XV, namely YOL155c, YOL154w, YOL119c, YOL118c, YOR301w and YOR306c, in FY1679 and CEN.PK2 backgrounds. We constructed replacement cassettes and cloned each ORF into the pRS416 centromeric plasmid. No obvious phenotype was observed for the corresponding deleted strains with respect to growth, mating or sporulation. YOL155c encodes a protein with a secretion signal and putative GPI-anchor recognition site and is possibly a cell wall protein, although its deletion did not present morphogenetic defects under any of the conditions tested. Although YOL119c and YOR306c are members of the monocarboxylate permease family, the growth of the double disruptant in acetate, lactate and pyruvate was similar to that of the parental strains.

📜 SIMILAR VOLUMES

Disruption of six novel ORFs fromSacchar
Disruption of six novel ORFs fromSaccharomyces cerevisiae chromosome VII and phenotypic analysis of the deletants
✍ Victoria Escribano, M.; Maz�n, Mar�a J. 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 309 KB 👁 2 views

We describe the disruption and basic phenotypic analysis of six open reading frames (ORFs) of unknown function located in the left arm of Saccharomyces cerevisiae chromosome VII, namely YGL133w, YGL134w, YGL136c, YGL138c, YGL142c and YGL144c. Disruptions were made using the short ¯anking homology PC

Functional analysis of 12 ORFs fromSacch
Functional analysis of 12 ORFs fromSaccharomyces cerevisiae chromosome II
✍ Rodríguez-Navarro, S.; Estruch, F.; Pérez-Ortin, J. E. 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 231 KB 👁 1 views

Twelve different ORFs have been deleted from the right arm of Saccharomyces cerevisiae chromosome II; namely YBR193c, YBR194w, YBR197c, YBR198c, YBR201w, YBR203w, YBR207w, YBR209w, YBR210w, YBR211c, YBR217w and YBR228w. Tetrad analysis of heterozygous deletant strains revealed that YBR193c, YBR198c

Disruption and phenotypic analysis of se
Disruption and phenotypic analysis of seven ORFs from the left arm of chromosome XV ofSaccharomyces cerevisiae
✍ Hajji, Khalid; Clotet, Josep; Ariño, Joaqu??ñ 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 249 KB 👁 1 views

We have disrupted seven open reading frames (ORFs) located in the left arm of chromosome XV of the yeast Saccharomyces cerevisiae. These ORFs, previously discovered by our laboratory during the programme of systematic sequencing of the yeast genome, are YOL152w, YOL151w, YOL149w, YOL130w, YOL128c, Y

Construction and genetic analysis ofS. c
Construction and genetic analysis ofS. cerevisiae deletants of six novel ORFs from chromosome II
✍ Malagón, Francisco; Aguilera, Andrés 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 223 KB 👁 1 views

We have constructed S. cerevisiae strains carrying genomic deletions of six ORFs from the left arm of chromosome II (YBL018c, YBL019w, YBL024w, YBL042c, YBL043w and YBL046w) in both FY1679 and W303 backgrounds. We have found that YBL018c is an essential gene in yeast, whereas the other five genes ar

Disruption of six novel genes from the l
Disruption of six novel genes from the left arm of chromosome XV ofSaccharomyces cerevisiae and basic phenotypic analysis of the generated mutants
✍ Séron, Karin; Blondel, Marie-Odile; Haguenauer-Tsapis, Rosine 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 242 KB 👁 2 views

Six open reading frames (ORFs) of unknown function from the left arm of Saccharomyces cerevisiae chromosome XV were deleted in two genetic backgrounds by disruption cassettes with long flanking homology (LFH) , within the frame of the research project EUROFAN. The LFH disruption cassettes, obtained

Disruption and basic phenotypic analysis
Disruption and basic phenotypic analysis of six novel genes from the left arm of chromosome XIV ofSaccharomyces cerevisiae
✍ Dueñas, Encarnación; Revuelta, José Luis; Del Rey, Francisco; Vázquez de Aldana, 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 337 KB 👁 1 views

We describe here the construction of six deletion mutants and their basic phenotypic analysis in three different backgrounds. The six genes were disrupted in three diploid strains (FY1679, W303 and CEN.PK2) by the long flanking homology (LFH) method (Wach, 1996). Transformants were selected as genet