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Disruption of six novel ORFs fromSaccharomyces cerevisiae chromosome VII and phenotypic analysis of the deletants

✍ Scribed by Victoria Escribano, M.; Maz�n, Mar�a J.

Publisher
John Wiley and Sons
Year
2000
Tongue
English
Weight
309 KB
Volume
16
Category
Article
ISSN
0749-503X

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✦ Synopsis

We describe the disruption and basic phenotypic analysis of six open reading frames (ORFs) of unknown function located in the left arm of Saccharomyces cerevisiae chromosome VII, namely YGL133w, YGL134w, YGL136c, YGL138c, YGL142c and YGL144c. Disruptions were made using the short ¯anking homology PCR replacement strategy in the FY1679 and CEN.PK2 diploid strains. Sporulation and tetrad analysis of the heterozygous deletants was performed, as well as phenotypic analysis of the corresponding deleted haploid strains. No obvious phenotypes could be attributed to the strains deleted in any of the genes YGL134w, YGL138c and YGL144c under the conditions tested. YGL142c was shown to be an essential gene. Segregants bearing a deletion in YGL136c grew slowly in complete glycerol medium at 37uC. Cells deleted in YGL133w showed abnormal morphology and reduced mating ef®ciency, but these phenotypes were observed only when the YGL133w disruption was in a MATa background. Ygl133 protein was found to localize to the nucleus.

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