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Disruption of sixSaccharomyces cerevisiae novel genes and phenotypic analysis of the deletants

✍ Scribed by Zúñiga, Sonia; Boskovic, Jasminka; Jiménez, Antonio; Ballesta, Juan P. G.; Remacha, Miguel

Publisher
John Wiley and Sons
Year
1999
Tongue
English
Weight
303 KB
Volume
15
Category
Article
ISSN
0749-503X

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✦ Synopsis

As a part of the EUROFAN programme, six open reading frames from Saccharomyces cerevisiae (YNL083w, YNL086w, YNL087w, YNL097c, YDL100c and YOR086c) were disrupted in two genetic backgrounds, FY1679 and W303. Individual deletions in diploid strains and tetrad analysis of heterozygous deletants revealed that none of them is essential. Basic phenotypic analysis did not reveal any significant difference between the parental and mutant strains. Although YNL087w and YOR086c are 55% identical, the double disruptant also behaves the same as the parental cells. Ydl100p seems to be involved in metal detoxification, the phenotype of the null mutants being enhanced when the assays are performed at 37 C.

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