Zeiss: Living Cells www.zeiss.de/FluoresScience Controlled Light Exposure Microscopy R.A. Hoebe and colleagues describe a method to reduce phototoxicity and photodamage to sensitive fluorescence samples. By spatially controlling the time of light exposure, the light-dose required to generate an imag
News Ticker: Imaging & Microscopy 3/2007
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 2007
- Weight
- 213 KB
- Volume
- 9
- Category
- Article
- ISSN
- 1439-4243
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โฆ Synopsis
present a study of the cause of several apparent resonance peaks shown by the probe resonance curve of the atomic force microscope contains, whose origin is not well understood, and try to find the optimal imaging conditions for acoustic intermittent contact mode in fluid environment. The authors demonstrate that the peaks are also present in the spectrum of the fluid movement and in that of the shaker piezo. These peaks may or may not coincide with the natural resonance of a probe in liquid, thus it is possible to drive the probes off-resonance. Numerical calculations show the feasibility of off-resonance imaging, but predict much higher imaging force.
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