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New possibilities of enzymic synthesis of radioactive nucleotides. I. Synthesis of radioactive nucleoside-5′-triphosphates by an enzyme of Escherichia coli B

✍ Scribed by Zdeněk Nejedlý; Jindřich Ekl; Karel Hybš; Jiří Filip

Publisher
John Wiley and Sons
Year
1969
Tongue
French
Weight
641 KB
Volume
5
Category
Article
ISSN
0022-2135

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✦ Synopsis

Possibilities of enzymic synthesis of nucleoside-.5'-triphosphates-14CC(U) of adenine, guanine, cytosine and uracil of high spec@ activity and radiochemical purity by means of a purijied enzyme isolatedfrom Escherichia coli B were studied. The efect of enzyme concentration in the reaction mixture in dependence on the time of the enzyme reaction is decisive for an optimal production of radioactive nucleoside-5'-triphosphates. A simple and rapid method of preparation of radioactive nucleoside-5'-triphosphates, including preparative paper chromatography as the single isolation step, is described and information on the radiolysis of the isolated nucleotides is presented.

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