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Synthesis of 14C-uridine-5′ -triphosphate by enzymes of escherichia coli cell-free extracts

✍ Scribed by P. Pumpen; E. Gren

Publisher
John Wiley and Sons
Year
1972
Tongue
French
Weight
379 KB
Volume
8
Category
Article
ISSN
0022-2135

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✦ Synopsis

Abstract

Crude cell‐free extracts of Escherichia coli AB 259 Hfr 3000, thi‐perform multistep conversion of ^14^C‐uracil to ^14^C‐UTP in vitro in the presence of D‐ribose/or ribose‐5‐phosphate/, ATP and ATP‐regenerating system. ATP concentration is crucial for significant ^14^C‐UTP production. Quantitative yield of the product combined with relatively simple procedure and inexpensiveness of initial materials makes the method advantageous for large‐scale preparation of ^14^C‐UTP.

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