✦ LIBER ✦

Synthesis of [14C]coproporphyrin III by yeast cell-free extracts

✍ Scribed by Henriette Chambon; Pierre Labbe

Publisher: Elsevier Science
Year: 1982
Tongue: English
Weight: 433 KB
Volume: 126
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(82)90111-7

No coin nor oath required. For personal study only.

✦ Synopsis

The procedure described utilizes a crude cell-free extract from the yeast Saccharomyces cerevisiae as enzymatic source for the synthesis of coproporphyrin III from [r4C]G-aminolevulinic acid with a high yield of conversion (-60%). Roth specific radioactivity and total radioactivity of coproporphyrin III can be adjusted fairly well. This procedure is not time consuming for yeast acellular extracts or porphyrin ester preparations. The acellular extracts can be stored frozen (-3O'C) for at least 1 year without loss of enzymatic activity. The same procedure can be used for ['4C]protoporphyrin preparation.

📜 SIMILAR VOLUMES

Synthesis of 14C-uridine-5′ -triphosphat

Synthesis of 14C-uridine-5′ -triphosphate by enzymes of escherichia coli cell-free extracts

✍ P. Pumpen; E. Gren 📂 Article 📅 1972 🏛 John Wiley and Sons 🌐 French ⚖ 379 KB

## Abstract Crude cell‐free extracts of Escherichia coli AB 259 Hfr 3000, thi‐perform multistep conversion of ^14^C‐uracil to ^14^C‐UTP in vitro in the presence of D‐ribose/or ribose‐5‐phosphate/, ATP and ATP‐regenerating system. ATP concentration is crucial for significant ^14^C‐UTP production. Qu

Effect of certain tetracycline analogs o

Effect of certain tetracycline analogs on phenylalanine-14C incorporation by Escberichia coli B cell-free extracts

✍ Carl B. Rifino; William F. Bousquet; Adelbert M. Knevel; Patrick Belcastro; Alfr 📂 Article 📅 1968 🏛 John Wiley and Sons 🌐 English ⚖ 258 KB

## I1 in relation to the caffeine molecule. This arrangement is consistent with the ideas presented above for polarization bonding. A more detailed account of this material will be published at a later date. In conclusion, it is felt that in order to propose molecular models for xanthine complexe

ChemInform Abstract: The Efficient Synth

ChemInform Abstract: The Efficient Synthesis of 14-Alkyl or Aryl 14H-Dibenzo[a,j]xanthenes Catalyzed by Bismuth(III) Chloride under Solvent-Free Conditions.

✍ Ebrahim Soleimani; Mohammad Mehdi Khodaei; Afsaneh Taheri Kal Koshvandi 📂 Article 📅 2011 🏛 John Wiley and Sons ⚖ 26 KB 👁 1 views

New possibilities of enzyme synthesis of

New possibilities of enzyme synthesis of radioactive nucleotides. II. Phosphoribosylation of radioactive bases of nucleic acids by the catalytic effect of unpurified cell-free extract of Brevibacterium ammoniagenes

✍ Zdeněk Nejedlý; Helena Škodová; Karel Hybš; Jan Škoda 📂 Article 📅 1970 🏛 John Wiley and Sons 🌐 French ⚖ 473 KB