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NEW Possibilities of preparative synthesis of radioactive nucleotides V. Enzyme synthesis of tritium labelled uridine 5′-triphosphate in high yield

✍ Scribed by J. Filip; L. Bohacek; J. Vesely; A. Cihak

Publisher
John Wiley and Sons
Year
1974
Tongue
French
Weight
248 KB
Volume
10
Category
Article
ISSN
0022-2135

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✦ Synopsis

Abstract

Growth of Escherichia coli B in the presence of sub−bacteriostatic concentration of various compounds results in the enhancement of activity of enzymes catalyzing the transformation of uridine to its 5′‐phosphates. Partially purified extracts of E. coli cultivated in the presence of 1×10^−5^M 5‐azacytidine were applied for the preparative synthesis of tritium‐labelled uridine 5′‐triphosphate. Yield of the newly formed nucleotide was about 90% and was higher during the short‐term incubation using enzyme extract from the treated cells than that from controls.

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