New possibilities of enzymic synthesis of radioactive nucleotides. IV. Application of in vivo tryptophan-stabilized enzyme system from regenerating rat liver for the synthesis of radioactive thymidine 5′ - phosphates in vitro
✍ Scribed by Jiri Filip; Karel Hybs; Zdenek Nejedly; Januaz Greger; Jiri Vesely; Alois Cihak
- Publisher
- John Wiley and Sons
- Year
- 1972
- Tongue
- French
- Weight
- 313 KB
- Volume
- 8
- Category
- Article
- ISSN
- 0022-2135
No coin nor oath required. For personal study only.
✦ Synopsis
The e n z y m a t i c e x t r a c t p t e p a r e d f r o m 48h. r e g e n e r at i n g r a t l i v e r s t a b i l i z e d w i t h d i e t a r y L -t r y p t o p h a n p o s s e s s e s t h e enhanced t h y m i d i n e and t h y m i d y l a t e k inaae a c t i v i t y . The o p t i m a l c o n d i t i o n 8 f o r t h e phoap h o r y l a t i o n o f t h y m i d i n e in vitro were f o u n d and the p O 8 8 i b i Z i t y f o r t h e p r e p a r a t i v e u s e 6 was c o n s i d e r e d . The amount of newly formed t h y m i d i n e 5'-monophosphate and S ' -t r i p h o s p h a t e ddpends on t h e l e v e l o f a d e n o s i n e