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New possibilities of enzymic synthesis of radioactive nucleotides. III. Preparative synthesis of radioactive 5′-nucleotides by an unpurified cell-free extract of mouse leukemic cells

✍ Scribed by Z. Nejedlý; S. Hrušková; K. Hybσ; J. Filip; A. Cihák; J. Veselý

Publisher
John Wiley and Sons
Year
1971
Tongue
French
Weight
511 KB
Volume
7
Category
Article
ISSN
0022-2135

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✦ Synopsis

A K R leukemic mouse cells possess a considerable uridine X-inase activity. The ability of unpurijied cell-fvee extracts prepared .from these cells to phosphorylate nucleosides-ldC was investigated. The substrate specijicity of the enzyme preparation and the possibility to regulate the course of the enzymatic reactions were examined. The method is convenient for the preparative synthesis of uracil, cytosine and hypoxanthine S-n~cleotides-~~C.

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## Abstract Growth of __Escherichia coli__ B in the presence of sub−bacteriostatic concentration of various compounds results in the enhancement of activity of enzymes catalyzing the transformation of uridine to its 5′‐phosphates. Partially purified extracts of __E. coli__ cultivated in the presenc