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Methacryloylamidohistidine in affinity ligands for immobilized metal-ion affinity chromatography of ferritin

✍ Scribed by Deniz Aktaş Uygun; Nevra Öztürk; Sinan Akgöl; Adil Denizli

Book ID
107638075
Publisher
The Korean Society for Biotechnology and Bioengineering
Year
2011
Tongue
English
Weight
746 KB
Volume
16
Category
Article
ISSN
1226-8372

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✍ Federico Maisano; Silvia Astrua Testori; Guido Grandi 📂 Article 📅 1989 🏛 Elsevier Science 🌐 English ⚖ 318 KB

Affinity chromatography on immobilized metal ions (IMAC) is a separation technique that takes advantage of the different capabilities of proteins of interacting with metal ions mainly through their exposed histidine residues. The metal ions are immobilized on suitable supports through a chelating li