Isolation of lactoferrin by immobilized metal ion affinity chromatography (IMAC)
β Scribed by Alejandra Calvo; Francisco Batista-Viera
- Publisher
- Elsevier Science
- Year
- 1994
- Tongue
- English
- Weight
- 353 KB
- Volume
- 22
- Category
- Article
- ISSN
- 0307-4412
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π SIMILAR VOLUMES
Phosphoproteins and phosphoamino acids bind to ferric ions immobilized on iminodiacetate-agarose gel and can be eluted by increasing pH or by introducing phosphate ions to the eluant. Some other metals were found to resemble iron with regard to the interaction with protein-bound phosphate and phosph
Affinity chromatography on immobilized metal ions (IMAC) is a separation technique that takes advantage of the different capabilities of proteins of interacting with metal ions mainly through their exposed histidine residues. The metal ions are immobilized on suitable supports through a chelating li
## Abstract A method based on Immobilised Metal Ion Affinity Chromatography (IMAC) using a chelating resin (Spheron Oxine 1000) loaded with Pd(II) is evaluated for the extraction and preconcentration of glyphosate and AMPA from natural water samples. The efficiency of the metalβloaded resin in reta
Electrospray ionization mass spectrometry (ESI/MS) affords a rapid and sensitive technique for determining peptides produced by the enzymatic digestion of phosphoroteins. When coupled with on-line immobilized metal-ion affinity chromatography (IMAC), the combmation allows separation and mass spectro