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Immobilized metal-ion affinity chromatography of peptides on metalloporphyrin stationary phases

โœ Scribed by Magdalena Biesaga; Jowita Orska; Dariusz Fiertek; J. Izdebski; M. Trojanowicz


Publisher
Springer
Year
1999
Tongue
English
Weight
109 KB
Volume
364
Category
Article
ISSN
1618-2650

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The separation of more than 30 biologically active synthetic peptides and their analogs on a high-performance immobilized metal ion affinity chromatography column is described. The metal chelating gel (TSK gel chelate-5PW) contains iminodiacetic acid (IDA) covalently coupled to a hydrophilic, resin-

Immobilized metal-ion affinity chromatog
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Affinity chromatography on immobilized metal ions (IMAC) is a separation technique that takes advantage of the different capabilities of proteins of interacting with metal ions mainly through their exposed histidine residues. The metal ions are immobilized on suitable supports through a chelating li