Affinity chromatography on immobilized metal ions (IMAC) is a separation technique that takes advantage of the different capabilities of proteins of interacting with metal ions mainly through their exposed histidine residues. The metal ions are immobilized on suitable supports through a chelating li
โฆ LIBER โฆ
Immobilization of silver and platinum ions for metal affinity chromatography
โ Scribed by A.A. Garcia; D.H. Kim; D.R. Miles
- Publisher
- Elsevier Science
- Year
- 1994
- Weight
- 659 KB
- Volume
- 23
- Category
- Article
- ISSN
- 0923-1137
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This work demonstrates that proper selection of a metal ion and chelating ligand enables recovery of a his(6)-tagged protein from canola (Brassica napus) extracts by immobilized metal affinity chromatography (IMAC). When using Co(2+) with iminodiacetate (IDA) as the chelating ligand, beta-glucuronid