Affinity chromatography on immobilized metal ions (IMAC) is a separation technique that takes advantage of the different capabilities of proteins of interacting with metal ions mainly through their exposed histidine residues. The metal ions are immobilized on suitable supports through a chelating li
β¦ LIBER β¦
Methacrylamidohistidine in affinity ligands for immobilized metal-ion affinity chromatography of human serum albumin
β Scribed by Mehmet Odaba; Bora Garipcan; Semir Dede; Adil Denizli
- Publisher
- The Korean Society for Biotechnology and Bioengineering
- Year
- 2001
- Tongue
- English
- Weight
- 598 KB
- Volume
- 6
- Category
- Article
- ISSN
- 1226-8372
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## Abstract A new chelating compound has been developed for use in the immobilized metal affinity chromatographic (IMAC) separation of proteins. The bidentate ligand, Ξ±βamino phenylalanine tetrazole, **4**, was synthesized __via__ a fiveβstep synthesis from __N__βfluorenylmethoxycarbonyl phenylalan