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Affinity Chromatography Volume 578 || Immobilized Metal Ion Affinity Chromatography of Native Proteins

✍ Scribed by Zachariou, Michael


Book ID
115493186
Publisher
Humana Press
Year
2008
Weight
310 KB
Category
Article
ISBN
1597455822

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Immobilized metal-ion affinity chromatog
✍ Federico Maisano; Silvia Astrua Testori; Guido Grandi 📂 Article 📅 1989 🏛 Elsevier Science 🌐 English ⚖ 318 KB

Affinity chromatography on immobilized metal ions (IMAC) is a separation technique that takes advantage of the different capabilities of proteins of interacting with metal ions mainly through their exposed histidine residues. The metal ions are immobilized on suitable supports through a chelating li