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Immobilized Metal‐Ion Affinity Chromatography: Status and Trends

✍ Scribed by Gutiérrez, R.; Martín del Valle, E. M.; Galán, M. A.


Book ID
118129316
Publisher
Taylor and Francis Group
Year
2007
Tongue
English
Weight
863 KB
Volume
36
Category
Article
ISSN
1542-2119

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Immobilized metal-ion affinity chromatog
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Affinity chromatography on immobilized metal ions (IMAC) is a separation technique that takes advantage of the different capabilities of proteins of interacting with metal ions mainly through their exposed histidine residues. The metal ions are immobilized on suitable supports through a chelating li