Chelating peptide-immobilized metal ion affinity chromatography
β Scribed by M.C. Smith; T.C. Furman; J.A. Cook; T. Ingolia; H. Hsiung
- Book ID
- 107901243
- Publisher
- Elsevier Science
- Year
- 1989
- Tongue
- English
- Weight
- 69 KB
- Volume
- 36
- Category
- Article
- ISSN
- 0162-0134
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π SIMILAR VOLUMES
Affinity chromatography on immobilized metal ions (IMAC) is a separation technique that takes advantage of the different capabilities of proteins of interacting with metal ions mainly through their exposed histidine residues. The metal ions are immobilized on suitable supports through a chelating li
The interaction of immobilized metal-chelating adsorbents with a dual heterobifunctional soluble polyethylene glycol (PEG) of the form X-PEG-Y is described, where X represents an affinity ligand and Y a chelating agent. The bifunctional PEG derivative used in this study was biotin-PEG-iminodiacetic