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Perspectives of immobilized-metal affinity chromatography

✍ Scribed by Vladka Gaberc-Porekar; Viktor Menart


Book ID
117331139
Publisher
Elsevier Science
Year
2001
Tongue
English
Weight
245 KB
Volume
49
Category
Article
ISSN
0165-022X

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Immobilized metal affinity chromatograph
✍ Cheryl L. Flurer; Milos Novotny πŸ“‚ Article πŸ“… 1992 πŸ› John Wiley and Sons 🌐 English βš– 397 KB

## Abstract Fused silica capillaries of 250 ΞΌm i.d. were slurry‐packed with a TSK Chelate‐5PW stationary phase. Cu(II) was immobilized on the iminodiacetate surface, and proteins were separated using either an increasing imidazole gradient or a decreasing pH gradient. The microcolumn system increas