Relatively high levels of nutrients and low levels of waste products were maintained in cultures of hybridoma cells by dialysis against growth medium supplemented with fetal calf serum (FCS). This resulted in a greater than ten fold increase in cell density and monoclonal antibody titre compared to
Metabolism of hybridoma cells and antibody secretion at high cell densities in dialysis tubing
✍ Scribed by Claudia Käsehagen; Fritjof Linz; Gerlinde Kretzmer; Thomas Scheper; Karl Schügerl
- Publisher
- Elsevier Science
- Year
- 1991
- Tongue
- English
- Weight
- 802 KB
- Volume
- 13
- Category
- Article
- ISSN
- 0141-0229
No coin nor oath required. For personal study only.
📜 SIMILAR VOLUMES
The death rate of hybridoma cells, grown in a continuous culture, has been studied in a small air-lift loop reactor as a function of reactor height and injected gas flow rate. The first-order death-rate constant was found to be proportional to the reciprocal height and to the gas flow rate, in accor
Fusion of lymphoblastoid cell lines that produce human monoclonal antibodies against Pseudomonas aeruginosa with the human/mouse heteromyeloma SHM-D33 generated heterohybrids that were stable and secreted antibody in the range of 20 to 300 micrograms/ml. One of the hybridoma cell lines ws adapted to