𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Metabolism of hybridoma cells and antibody secretion at high cell densities in dialysis tubing

✍ Scribed by Claudia Käsehagen; Fritjof Linz; Gerlinde Kretzmer; Thomas Scheper; Karl Schügerl


Publisher
Elsevier Science
Year
1991
Tongue
English
Weight
802 KB
Volume
13
Category
Article
ISSN
0141-0229

No coin nor oath required. For personal study only.


📜 SIMILAR VOLUMES


In vitroproduction of high titre monoclo
✍ S. R. Adamson; S. L. Fitzpatrick; L. A. Behie; G. M. Gaucher; B. H. Lesser 📂 Article 📅 1983 🏛 Springer Netherlands 🌐 English ⚖ 252 KB

Relatively high levels of nutrients and low levels of waste products were maintained in cultures of hybridoma cells by dialysis against growth medium supplemented with fetal calf serum (FCS). This resulted in a greater than ten fold increase in cell density and monoclonal antibody titre compared to

Effect of serum concentration on hybrido
✍ D. E. Martens; E. C. Beuvery; C. D. De Gooijer; J. Tramper 📂 Article 📅 1992 🏛 John Wiley and Sons 🌐 English ⚖ 688 KB

The death rate of hybridoma cells, grown in a continuous culture, has been studied in a small air-lift loop reactor as a function of reactor height and injected gas flow rate. The first-order death-rate constant was found to be proportional to the reciprocal height and to the gas flow rate, in accor

Heterohybridomas that secrete high level
✍ Maureen C. Gammon; Martin H. Banas; Louis E. Boccumini; Nolan H. Sigal; Hans J. 📂 Article 📅 1990 🏛 Springer 🌐 English ⚖ 821 KB

Fusion of lymphoblastoid cell lines that produce human monoclonal antibodies against Pseudomonas aeruginosa with the human/mouse heteromyeloma SHM-D33 generated heterohybrids that were stable and secreted antibody in the range of 20 to 300 micrograms/ml. One of the hybridoma cell lines ws adapted to