To investigate the response of hybridoma cells to hypoosmotic stress, S3H5/β₯2bA2 and DB9G8 hybridomas were cultivated in the hypoosmolar medium [Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% serum] resulting from sodium chloride subtraction. Both hybridomas showed similar responses
Role of glutamine in hybridoma cell culture: Effects on cell growth, antibody production, and cell metabolism
β Scribed by Yeon-Ho Jeong; Shaw S. Wang
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- English
- Weight
- 988 KB
- Volume
- 17
- Category
- Article
- ISSN
- 0141-0229
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## Abstract The flowβcytometric (FCM) analysis of bivariate DNA/lgG distributions has been conducted to study the cell cycle kinetics and monoclonal antibody (MAb) production during perfusion culture of hybridoma cells. Three different perfusion rates were employed to demonstrate the dependency of