## Abstract **BACKGROUND:** The production of Monoclonal antibodies (mAbs) is often performed in batch or fedβbatch operations where low cell densities and low volumetric productivities are achieved. The main bottleneck of both processes is the short operating time with productive cells at maximum
The loss of antibody productivity in continuous culture of hybridoma cells
β Scribed by Kelly K. Frame; Wei-Shou Hu
- Publisher
- John Wiley and Sons
- Year
- 1990
- Tongue
- English
- Weight
- 722 KB
- Volume
- 35
- Category
- Article
- ISSN
- 0006-3592
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An immunoglobulin G (lgG2b) producing hybridoma cell line (S3H5/y2bA2) was cloned and subcloned. Twenty subclones were grown in parallel while being adapted in a stepwise fashion to serum-free medium. Following adaptation to serum-free medium, it was found that 16 of the 20 subclones remained at a r
## Abstract The flowβcytometric (FCM) analysis of bivariate DNA/lgG distributions has been conducted to study the cell cycle kinetics and monoclonal antibody (MAb) production during perfusion culture of hybridoma cells. Three different perfusion rates were employed to demonstrate the dependency of
## Several analytical techniques were applied to obtain information about the stability of expression, yield and integrity of monoclonal antibodies (Mabs) produced by hybridoma cells in homogeneous continuous perfusion culture systems. An isotype-specific spot enzyme-linked immunosorbent assay (sp
## Abstract **BACKGROUND:** Many reports have suggested that mild hypothermic culture conditions improve the specific monoclonal antibody (mAb) productivity of mammalian cells. The effect of active hypothermic growth on the mAb productivity of the hybridoma C~2~E~7~ was investigated. Hybridoma grow