𝔖 Bobbio Scriptorium
✦   LIBER   ✦

The loss of antibody productivity in continuous culture of hybridoma cells

✍ Scribed by Kelly K. Frame; Wei-Shou Hu


Publisher
John Wiley and Sons
Year
1990
Tongue
English
Weight
722 KB
Volume
35
Category
Article
ISSN
0006-3592

No coin nor oath required. For personal study only.


πŸ“œ SIMILAR VOLUMES


Continuous perfusion culture of encapsul
✍ MartΓ­ Lecina; Albert TintΓ³; Jordi GΓ‘lvez; Francesc GΓ²dia; Jordi J. CairΓ³ πŸ“‚ Article πŸ“… 2011 πŸ› Wiley (John Wiley & Sons) 🌐 English βš– 385 KB

## Abstract **BACKGROUND:** The production of Monoclonal antibodies (mAbs) is often performed in batch or fed‐batch operations where low cell densities and low volumetric productivities are achieved. The main bottleneck of both processes is the short operating time with productive cells at maximum

Loss of antibody productivity is highly
✍ Steven E. Merritt; Bernhard O. Palsson πŸ“‚ Article πŸ“… 1993 πŸ› John Wiley and Sons 🌐 English βš– 288 KB

An immunoglobulin G (lgG2b) producing hybridoma cell line (S3H5/y2bA2) was cloned and subcloned. Twenty subclones were grown in parallel while being adapted in a stepwise fashion to serum-free medium. Following adaptation to serum-free medium, it was found that 16 of the 20 subclones remained at a r

Cell cycle kinetics and monoclonal antib
✍ Sun Ho Park; Dewey D. Y. Ryu πŸ“‚ Article πŸ“… 1994 πŸ› John Wiley and Sons 🌐 English βš– 565 KB

## Abstract The flow‐cytometric (FCM) analysis of bivariate DNA/lgG distributions has been conducted to study the cell cycle kinetics and monoclonal antibody (MAb) production during perfusion culture of hybridoma cells. Three different perfusion rates were employed to demonstrate the dependency of

Methods for studying the stability of an
✍ Jose M Coco-Martin; Jan W Oberink; Tiny A.M van der Velden-de Groot; E.Coen Beuv πŸ“‚ Article πŸ“… 1991 πŸ› Elsevier Science 🌐 English βš– 495 KB

## Several analytical techniques were applied to obtain information about the stability of expression, yield and integrity of monoclonal antibodies (Mabs) produced by hybridoma cells in homogeneous continuous perfusion culture systems. An isotype-specific spot enzyme-linked immunosorbent assay (sp

Enhancement of monoclonal antibody produ
✍ Sun Li Chong; Duen Gang Mou; Saw Hoon Lim; Abdul Manaf Ali; Beng Ti Tey πŸ“‚ Article πŸ“… 2009 πŸ› Wiley (John Wiley & Sons) 🌐 English βš– 135 KB

## Abstract **BACKGROUND:** Many reports have suggested that mild hypothermic culture conditions improve the specific monoclonal antibody (mAb) productivity of mammalian cells. The effect of active hypothermic growth on the mAb productivity of the hybridoma C~2~E~7~ was investigated. Hybridoma grow