Loss of antibody productivity is highly reproducible in multiple hybridoma subclones

✍ Scribed by Steven E. Merritt; Bernhard O. Palsson

• Publisher: John Wiley and Sons
• Year: 1993
• Tongue: English
• Weight: 288 KB
• Volume: 42
• Category: Article
• ISSN: 0006-3592
• DOI: 10.1002/bit.260420213

✦ Synopsis

An immunoglobulin G (lgG2b) producing hybridoma cell line (S3H5/y2bA2) was cloned and subcloned. Twenty subclones were grown in parallel while being adapted in a stepwise fashion to serum-free medium. Following adaptation to serum-free medium, it was found that 16 of the 20 subclones remained at a relatively constant proportion of nonproducing cells. Three of the remaining subclones transiently deviated from this balance but eventually returned toward this population composition. One subclone continued to lose productivity. A population balance was reached at approximately 8% of the population being nonproducers. The loss of antibody productivity was thus highly reproducible.