Several experimental data on continuous cultures of hybridoma cells show that monoclonal antibody productivity is a decreasing function of dilution rate. It has been suggested that this unusual behavior may be due to the arrest of a fraction of cycling cells at a critical point of Phase GI. Although
Cultivation of hybridoma cells in continuous cultures: Kinetics of growth and product formation
β Scribed by N. G. Ray; S. B. Karkare; P. W. Runstadler Jr.
- Publisher
- John Wiley and Sons
- Year
- 1989
- Tongue
- English
- Weight
- 556 KB
- Volume
- 33
- Category
- Article
- ISSN
- 0006-3592
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## Abstract The kinetic behavior of a nonproducing hybridoma clone AFPβ27βNP was investigated in continuous culture under glucoseβlimited conditions. A total of more than 21, 000 h of cultures were operated at dilution rates ranging from 0.01 to 0.06 h^β1^. The viable cell concentrations, dead cell
A hybridoma cell line, AFP-27-P, was cultivated in continuous culture under glucose-limited conditions. The viable cell concentration, dead-cell concentration, and cell volume all varied with the dilution rate. A model previously developed for a nonproducing clone of the same cell line, AFP-27-NP, w