Hybridoma growth and antibody production as a function of cell density and specific growth rate in perfusion culture
โ Scribed by Gautam G. Banik; Carole A. Heath
- Publisher
- John Wiley and Sons
- Year
- 1995
- Tongue
- English
- Weight
- 1014 KB
- Volume
- 48
- Category
- Article
- ISSN
- 0006-3592
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๐ SIMILAR VOLUMES
A quantitative study of the influence of initial serum concentration on hybridoma growth rate, maximum viable and total cell yield, and specific antibody production rate is presented. The specific growth rate varied in a Monod fashion with initial serum levels (2-10ยฐ/0 FCS), giving K,,, = 1.6 v/v% a
Experimental data from six hybridoma cell lines grown under diverse experimental conditions in both normal continuous and perfusion cultures are analyzed with respect to the significance of nutrients and products in determining the growth and death rates of cells and with respect to their mathematic