Heterohybridomas that secrete high levels of pseudomonas-specific therapeutic human monoclonal antibodies: their generation and large scale growth in an automated hollow fiber cell culture system
✍ Scribed by Maureen C. Gammon; Martin H. Banas; Louis E. Boccumini; Nolan H. Sigal; Hans J. Zweerink
- Publisher
- Springer
- Year
- 1990
- Tongue
- English
- Weight
- 821 KB
- Volume
- 3
- Category
- Article
- ISSN
- 0920-9069
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✦ Synopsis
Fusion of lymphoblastoid cell lines that produce human monoclonal antibodies against Pseudomonas aeruginosa with the human/mouse heteromyeloma SHM-D33 generated heterohybrids that were stable and secreted antibody in the range of 20 to 300 micrograms/ml. One of the hybridoma cell lines ws adapted to serum-free medium and maintained for 60 days in an automated hollow fiber system. During that time, 3 g of antibody was produced. Such yields make it possible to evaluate these monoclonals for their therapeutic potential in patients at risk for Pseudomonas infections.