Metabolic kinetics of nonproductive binding inhibition
β Scribed by Tomoo Funaki; Hideo Fukazawa; Isami Kuruma
- Book ID
- 102413374
- Publisher
- John Wiley and Sons
- Year
- 1994
- Tongue
- English
- Weight
- 269 KB
- Volume
- 83
- Category
- Article
- ISSN
- 0022-3549
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
Compounds similar in structure to reactants, intermediates and products of the aldolase-catalysed reaction were synthesized and their affinities for the enzyme determined. The best situations were found with bdicarbonyl phosphorylated compounds which are a good mimics of the incoming groups in the b
A general procedure is described for determining the kinetic constants of the slow, tight-binding inhibition of enzyme-catalyzed reactions by analyzing the data of initial and steady-state rate. All unknown parameters can be determined from several simple, sequential calculations. This method is sim