✦ LIBER ✦

A Simple Method for Determining Kinetic Constants of Slow, Tight-Binding Inhibition

✍ Scribed by Z.X. Wang

Publisher: Elsevier Science
Year: 1993
Tongue: English
Weight: 467 KB
Volume: 213
Category: Article
ISSN: 0003-2697
DOI: 10.1006/abio.1993.1434

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✦ Synopsis

A general procedure is described for determining the kinetic constants of the slow, tight-binding inhibition of enzyme-catalyzed reactions by analyzing the data of initial and steady-state rate. All unknown parameters can be determined from several simple, sequential calculations. This method is simple and rigorous. It is also applicable to the special case of slow-binding inhibition, where the total concentration of inhibitor is much higher than that of the enzyme. 1983 Academic Press, Inc.

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