𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Kinetic and spectroscopic study of slow-binding inhibition processes in aldolase

✍ Scribed by C. Blonski; T. Gefflaut; J. Perie

Publisher
John Wiley and Sons
Year
1998
Tongue
English
Weight
120 KB
Volume
11
Category
Article
ISSN
0894-3230

No coin nor oath required. For personal study only.

✦ Synopsis

Compounds similar in structure to reactants, intermediates and products of the aldolase-catalysed reaction were synthesized and their affinities for the enzyme determined. The best situations were found with bdicarbonyl phosphorylated compounds which are a good mimics of the incoming groups in the bond-forming process; the corresponding binding is characterized by slow-binding inhibition type, the inhibitors forming stabilized iminium ions and enamines with the enzyme; similar effects were obtained with an aromatic aldehyde, also capable of forming a stabilized iminium ion. The use of aldolase mutants allows one to characterize the lysyl group involved in the process and also to suggest a proton transfer mechanism for the iminium ion formation with the enzyme natural substrate.

πŸ“œ SIMILAR VOLUMES

An in situ Raman spectroscopic study of
An in situ Raman spectroscopic study of electrochemical processes in mercury–solution interphases
✍ Alexandre G. Brolo; Marek Odziemkowski; Donald E. Irish πŸ“‚ Article πŸ“… 1998 πŸ› John Wiley and Sons 🌐 English βš– 299 KB πŸ‘ 2 views

Electrochemical processes in the mercury-solution interphase were probed by in situ spectroelectrochemical micro-Raman spectroscopy. This is the Ðrst report of the in situ characterization of electrochemical processes for a pure mercury electrode by Raman spectroscopy. Mercury was oxidized in 0.1 M

Origin of the Slow-Binding Inhibition of
Origin of the Slow-Binding Inhibition of Aldolase by D-glycero-Tetrulose 1-Phosphate (D-Erythrulose 1-Phosphate) from the Comparison with the Isosteric Phosphonate Analog
✍ Patrick Page; Casimir Blonski; Jacques PΓ©riΓ© πŸ“‚ Article πŸ“… 1999 πŸ› John Wiley and Sons 🌐 English βš– 246 KB

The mechanistic reaction pathway for the slow-binding enamine intermediate. We show from these results that enzyme slow-binding inhibition by D-erythrulose 1-inhibition of rabbit muscle aldolase by D-glycero-tetrulose 1phosphate (D-erythrulose 1-phosphate) was investigated phosphate is consistent wi

In situ fluorescence study of slow relea
In situ fluorescence study of slow release and swelling processes in gels formed by solution free radical copolymerization
✍ Pekcan, Γ–.; Yilmaz, Y.; Uğur, Ş. πŸ“‚ Article πŸ“… 1997 πŸ› John Wiley and Sons 🌐 English βš– 408 KB πŸ‘ 2 views

A novel method based on steady state Γ‘uorescence (SSF) measurements is introduced for studying slow release and swelling processes in gels formed by free radical crosslinking copolymerization (FCC) of methyl methacrylate (MMA) and ethylene glycol dimethacrylate (EGDM) in toluene. Gels were prepared