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Measurement of one-bond 13Cα–1Hα residual dipolar coupling constants in proteins by selective manipulation of CαHα spins

✍ Scribed by Graeme Ball; Nicola Meenan; Krystyna Bromek; Brian O. Smith; Juraj Bella; Dušan Uhrín

Book ID
108184759
Publisher
Elsevier Science
Year
2006
Tongue
English
Weight
558 KB
Volume
180
Category
Article
ISSN
1090-7807

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Simultaneous measurement of NH and CαHα
Simultaneous measurement of NH and CαHα coupling constants in proteins
✍ David Pantoja-Uceda; Jorge Santoro 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 English ⚖ 241 KB

## Abstract We present a pulse sequence for the simultaneous measurement of NH and CαHα couplings in double‐labeled proteins from 2D spectra. The proposed sequence, a modification of the HN(CO)CA experiment, combines the __J__‐modulation method and the IPAP scheme. The couplings can be readily re