An HNCO-based Pulse Scheme for the Measurement of 13Cα-1Hα One-bond Dipolar couplings in 15N, 13C Labeled Proteins
✍ Scribed by Daiwen Yang; Joel R. Tolman; Natalie K. Goto; Lewis E. Kay
- Book ID
- 110260379
- Publisher
- Springer Netherlands
- Year
- 1998
- Tongue
- English
- Weight
- 157 KB
- Volume
- 12
- Category
- Article
- ISSN
- 0925-2738
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A triple-resonance pulse sequence is presented for the quantitative measurement of 1 H ␣ -13 C ␣ single-bond couplings in 15 N, 13 C uniformly labeled proteins. This 1 J CH -modulated (HACACO)NH experiment yields 1 H N -15 N-correlated 2D spectra in which the amplitude of each peak is modulated by t
Four simple and sensitive HNCO-based methods for measurement of 1J(C'Calpha), 1J(NCalpha) and 2J(NCalpha) coupling constants in protein main chains are presented. Three of these experiments enable the simultaneous measurement of 1J(C'Calpha), 1J(NCalpha) and 2J(NCalpha) couplings. Exploitation of th