𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Determination of Hα,Hβand Hβ,C′ coupling constants in13C-labeled proteins

✍ Scribed by U. Eggenberger; Y. Karimi-Nejad; H. Thüring; H. Rüterjans; C. Griesinger

Publisher
Springer Netherlands
Year
1992
Tongue
English
Weight
430 KB
Volume
2
Category
Article
ISSN
0925-2738

No coin nor oath required. For personal study only.

📜 SIMILAR VOLUMES

Determination of HN,Hαand HN,C′ coupling
Determination of HN,Hαand HN,C′ coupling constants in13C,15N-labeled proteins
✍ R. Weisemann; H. Rüterjans; H. Schwalbe; J. Schleucher; W. Bermel; C. Griesinger 📂 Article 📅 1994 🏛 Springer Netherlands 🌐 English ⚖ 603 KB

Sensitive three-dimensional NMR experiments, based on the E.COSY principle, are presented for the measurement of the 3J(HN, H~) and 3J(HN,C') coupling constants in uniformly 13C-and 15N-labeled proteins. They employ gradient coherence selection in combination with the sensitivity enhancement method

Simultaneous measurement of NH and CαHα
Simultaneous measurement of NH and CαHα coupling constants in proteins
✍ David Pantoja-Uceda; Jorge Santoro 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 English ⚖ 241 KB

## Abstract We present a pulse sequence for the simultaneous measurement of NH and CαHα couplings in double‐labeled proteins from 2D spectra. The proposed sequence, a modification of the HN(CO)CA experiment, combines the __J__‐modulation method and the IPAP scheme. The couplings can be readily re

H(C)CH-COSY and (H)CCH-COSY Experiments
H(C)CH-COSY and (H)CCH-COSY Experiments for13C-Labeled Proteins in H2O Solution
✍ Kalle Gehring; Irena Ekiel 📂 Article 📅 1998 🏛 Elsevier Science 🌐 English ⚖ 354 KB

We present three experiments which serve to identify carbon and proton sidechain resonances in 13 C-labeled proteins. The first is an improvement on the previously published H(C)CH-COSY experiment and comprises the application of gradients for coherence selection and a reduction in the phase cycle.