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Determination of HN,Hαand HN,C′ coupling constants in13C,15N-labeled proteins

✍ Scribed by R. Weisemann; H. Rüterjans; H. Schwalbe; J. Schleucher; W. Bermel; C. Griesinger

Publisher: Springer Netherlands
Year: 1994
Tongue: English
Weight: 603 KB
Volume: 4
Category: Article
ISSN: 0925-2738
DOI: 10.1007/bf00175250

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✦ Synopsis

Sensitive three-dimensional NMR experiments, based on the E.COSY principle, are presented for the measurement of the 3J(HN, H~) and 3J(HN,C') coupling constants in uniformly 13C-and 15N-labeled proteins.

They employ gradient coherence selection in combination with the sensitivity enhancement method in HSQC-type spectra (Cavanagh et al., 1991;Palmer et al., 1991). In most cases, the two measured coupling constants unambiguously define the ~)-angle for protein structure determination. The method is applied to uniformly 13C,15N-labeled ribonuclease T L.

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