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A new 3D HN(CA)HA experiment for obtaining fingerprint HN-Hαcross peaks in15N- and13C-labeled proteins

✍ Scribed by Robert T. Clubb; V. Thanabal; Gerhard Wagner


Publisher
Springer Netherlands
Year
1992
Tongue
English
Weight
426 KB
Volume
2
Category
Article
ISSN
0925-2738

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A 3D NOESY-(HCACO)NH experiment is described that transfers NOEs from 1H alpha to the backbone 1Hn in the succeeding residue for detection. Using this strategy, NOEs involving 1H alpha protons that resonate exactly at the water frequency can be detected. NOEs from an overlapping 1H alpha proton that