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A 3D NOESY-(HCACO)NH experiment for the measurement of NOEs involving1Hαin13C-/15N-labeled proteins dissolved in H2O

✍ Scribed by Weixing Zhang; William H. Gmeiner

Publisher
Springer Netherlands
Year
1996
Tongue
English
Weight
223 KB
Volume
8
Category
Article
ISSN
0925-2738

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✦ Synopsis

A 3D NOESY-(HCACO)NH experiment is described that transfers NOEs from 1H alpha to the backbone 1Hn in the succeeding residue for detection. Using this strategy, NOEs involving 1H alpha protons that resonate exactly at the water frequency can be detected. NOEs from an overlapping 1H alpha proton that is attached to degenerate 13C alpha can also be resolved. The performance of this approach is demonstrated for the 13C-/15N-labeled Hck/SH2 dissolved in H2O.

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