We have prepared RNAs uniformly 13C-labeled in the ribose ring and with the H3',H4',H5'/H5" protons specifically replaced with deuterons (3',4',5'/5"-2H-13C, termed d4-13C ribose), which offers the advantage of spectral simplification without sacrifice of sensitivity for the remaining protons. A 3D
The 2D NMR Experiments H(C)CO2and HCCO2for Assignment and pH Titration of Carboxylate Groups in Uniformly15N/13C-Labeled Proteins
✍ Scribed by Maurizio Pellecchia; Hideo Iwai; Thomas Szyperski; Kurt Wüthrich
- Publisher
- Elsevier Science
- Year
- 1997
- Tongue
- English
- Weight
- 112 KB
- Volume
- 124
- Category
- Article
- ISSN
- 1090-7807
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