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Measurement of 2J(H,C)- and 3J(H,C)-coupling constants by α/β selective HC(C)H-TOCSY

✍ Scribed by Elke Duchardt; Christian Richter; Bernd Reif; Steffen J. Glaser; Joachim W. Engels; Christian Griesinger; Harald Schwalbe

Book ID: 110313509
Publisher: Springer Netherlands
Year: 2001
Tongue: English
Weight: 220 KB
Volume: 21
Category: Article
ISSN: 0925-2738
DOI: 10.1023/a:1012474811265

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## Abstract We present a pulse sequence for the simultaneous measurement of NH and CαHα couplings in double‐labeled proteins from 2D spectra. The proposed sequence, a modification of the HN(CO)CA experiment, combines the __J__‐modulation method and the IPAP scheme. The couplings can be readily re