Measurement of two-bond JCOHαcoupling constants in proteins uniformly enriched with13C
✍ Scribed by Geerten W. Vuister; Ad Bax
- Publisher
- Springer Netherlands
- Year
- 1992
- Tongue
- English
- Weight
- 317 KB
- Volume
- 2
- Category
- Article
- ISSN
- 0925-2738
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## Abstract We present a pulse sequence for the simultaneous measurement of NH and CαHα couplings in double‐labeled proteins from 2D spectra. The proposed sequence, a modification of the HN(CO)CA experiment, combines the __J__‐modulation method and the IPAP scheme. The couplings can be readily re
Four simple and sensitive HNCO-based methods for measurement of 1J(C'Calpha), 1J(NCalpha) and 2J(NCalpha) coupling constants in protein main chains are presented. Three of these experiments enable the simultaneous measurement of 1J(C'Calpha), 1J(NCalpha) and 2J(NCalpha) couplings. Exploitation of th
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